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Effect of abiotic factors on Armillaria growth

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Effect of abiotic factors on Armillaria growth

Lead scientist
Dr Liz Beal
Start date
2003
End date
2009
Keywords

temperature, pH, moisture, organic matter, Armilllaria, honey fungus, abiotic, humidity, rhizomorph

Benefits to gardeners

The project aims to provide gardeners with better advice for dealing with Armillaria root rot problems related to the current changing climate conditions.

The problem

Armillaria sp. is the most frequently identified fungus causing plant death in British gardens by the plant pathology department at RHS Garden Wisley.

The majority of cases are caused by A. mellea, however A. gallica is often found and occasionally A. ostoyae and A. cepistipes. Previous research has demonstrated that A. gallica is able to kill live plants but only those that are stressed by other factors, for example overcrowding or drought.

With potential changes in climate, plants may suffer stress and therefore become more susceptible to a wider range of Armillaria species.
 

Approach

Temperature effects on survival of rhizomorphs were carried out using 2cm lengths of rhizomorphs grown up from hazel inoculum plugs. These inocula were placed into petri dishes containing horticultural grit. The variables were: level of moisture during heating (wet/dry), duration of heat (2hrs/12hrs), and the type of inoculum.

Initial results show temperature appears to have more of an influence on A. gallica and A. mellea survival rates than humidity. At 50oC no A.mellea or A. gallica survived.

To study the effect of pH and soil moisture on survival a standard loam will be used. The inoculum used is hazel plugs infected with Armillaria species.

For the pH experiment the pH will be adjusted at either 5, 6, 7 or 8. The trays will be kept moist so the water content is 50% of field capacity.
For the soil moisture experiment the moisture content will be kept at 10%, 30%, 50%, 70%, 90% or 100% of field capacity. The rhizomorphs will be harvested and measured after three months in both experiments.
 

Further information

Advice on honey fungus

Read about the studies on the pathogenicity of Armillaria spp

Read about the identification of Armillaria spp using molecular methods

Read about the behaviour of severed rhizomorphs of Armillaria mellea and A. gallica in growing media

Read the Armillaria survey

Read about the growth and behaviour of Armillaria in mulches
 

References

Pérez Sierra A (2003) Systematics, diagnostics and epidemiology of the fungal genus Armillaria. PhD thesis, University of London

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